Based on evolutionarily-informed expectations of gene content of near-universal single-copy orthologs, the BUSCO metric is complementary to technical metrics like N50.
BUSCO was selected as one of the SIB Remarkable Outputs of 2021!
The latest BUSCO paper, describing the novelties introduced in BUSCO v4 and v5 and the new BUSCO datasets (*_odb10) are described here. If you've used these versions or datasets the correct citation would be:
Mosè Manni, Matthew R Berkeley, Mathieu Seppey, Felipe A Simão, Evgeny M Zdobnov, BUSCO Update: Novel and Streamlined Workflows along with Broader and Deeper Phylogenetic Coverage for Scoring of Eukaryotic, Prokaryotic, and Viral Genomes. Molecular Biology and Evolution, Volume 38, Issue 10, October 2021, Pages 4647–4654
The following protocol covers the various BUSCO running modes and workflows, BUSCO setup, guidelines to interpret the results, and additional analyses, e.g., for building phylogenomic trees and visualizing syntenies using BUSCO results:
Manni, M., Berkeley, M. R., Seppey, M., & Zdobnov, E. M. (2021). BUSCO: Assessing genomic data quality and beyond. Current Protocols, 1, e323. doi: 10.1002/cpz1.323
The BUSCO software is licensed under the MIT License.
The BUSCO datasets are licensed under the Creative Commons Attribution-NoDerivatives 4.0 International License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nd/4.0/ or send a letter to Creative Commons, PO Box 1866, Mountain View, CA 94042, USA.
Any use of these datasets for analyses in a publication or product must include the citation of the corresponding paper: https://doi.org/10.1093/molbev/msab199.
BUSCO is available as a conda package and as a Docker image. Both of these versions are ready to run out of the box. Alternatively, it is also possible to manually install BUSCO.
The BUSCO software directly downloads the necessary datasets, whether they are specified by the user or automatically selected by BUSCO.
To display all available datasets
You can also download them manually.