from QC to gene prediction and phylogenomics
BUSCO v5.0.0 is the current stable version!
Gitlab, a Conda package and Docker container are also available.
Based on evolutionarily-informed expectations of gene content of near-universal single-copy orthologs, BUSCO metric is complementary to technical metrics like N50.
Availability
- Git source code
- Docker container
- Conda package
New in v4
- Bacteria & archaea revised
- Auto-lineage selection
- Automated download of datasets
vs CheckM
- Scores eukaryotes and prokaryotes
- Can run on a laptop
- Better resolution, less overestimates
Obtain BUSCO
Full installation instructions are provided in the user guide.
BUSCO is available as a conda package and as a Docker image. Both of these versions are ready to run out of the box. Alternatively, it is also possible to manually install BUSCO.
The BUSCO software now directly downloads the necessary datasets, specified by the user or automatically selected.
To display all available datasets
busco --list-datasets
You can also download them manually and see a list with the number of marker genes.
Earlier versions: v4, v3, v2, v1
Documentation and support
User guide Issues boardCite us
Seppey M., Manni M., Zdobnov E.M. (2019) BUSCO: Assessing Genome Assembly and Annotation Completeness. In: Kollmar M. (eds) Gene Prediction. Methods in Molecular Biology, vol 1962. Humana, New York, NY. 2019 doi.org/10.1007/978-1-4939-9173-0_14. PMID:31020564
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